Control method: 1. Propagation and cultivation of virus-free seedlings. Breeding and cultivating virus-free seedlings is the most fundamental measure to control grape black wood disease. When cultivating virus-free seedlings, we mainly grasp the following steps: ① Cultivate rootstocks through planting. ② Heat treatment between stems and virus removal by tissue culture. ③ The virus was identified by enzyme-linked immunosorbent assay or reverse transcription polymerase chain reaction and indicator plant detection. (4) Establish a virus-free mother tree orchard and provide non-toxic propagation materials. ⑤ Establish virus-free seedlings, quickly cultivate virus-free seedlings through grafting and cutting, and supply them to production in time. 2. Plant quarantine. When transporting seedlings and propagation materials in disease-free areas or newly-built vineyards, we should attach great importance to or strictly implement the quarantine system to avoid the introduction and spread of viruses. 3. Eliminate central disease strains. Careful observation should be made in the vineyard, the diseased plants should be pulled out and destroyed in time, and the soil should be disinfected locally to kill poisonous nematodes.
The grape housekeeper replied.