Accurately weigh an appropriate amount of this product, add an appropriate amount of anhydrous ethanol (about 25mg roxithromycin and 5ml anhydrous ethanol), dissolve it, and make a solution containing 10 unit and 5 units per 10ml with phosphate buffer of pH7.8, and determine it according to the microbial assay method of antibiotics (China Pharmacopoeia 1995 Edition, Part II, Appendix A, two-dose method), and the medium is pH.
Verification method double dose method
Bacillus subtilis CMCC(B)6350 1
Medium pH 7.8 ~ 8.0
The high and low dose concentrations were 5μ/ml and 10μ/ml, respectively.
Culture conditions: temperature 35 ~ 37℃, time 65438 06 ~ 65438 08 hours.
It can inhibit protein synthesis in bacteria, and its antibacterial spectrum is similar to erythromycin. It has antibacterial effects on Helicobacter pylori, Treponema pallidum, some anaerobic bacteria, mycoplasma pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes, Moraxella catarrhalis, Neisseria gonorrhoeae, Haemophilus chancre, Chlamydia trachomatis and Toxoplasma encephalitis. Oral absorption is good, because this product is fat-soluble, and it is absorbed well when taken with milk. After 2 hours, the plasma concentration reached the peak, t1/2 =10.5-13.8 hours, and the tissues were widely distributed and excreted from feces and urine in the original form. Used for eye, nose, ear and urinary tract infections, sexually transmitted diseases, vaginitis, skin and soft tissues, oral infections and chlamydia pneumoniae infections caused by sensitive bacteria. Clinical treatment of helicobacter pylori infection in stomach.