Edc is used as an activator of carboxyl group in amide synthesis, as well as for activating phosphate group, cross-linking of protein with nucleic acid and preparation of immunoconjugates. When used, the pH range is 4.0-6.0, and it is often used in combination with N- hydroxysuccinimide (NHS) or N- hydroxythiosuccinimide to improve the coupling efficiency.

In organic chemistry, EDC and catalyst 4- dimethylaminopyridine (DMAP) are used in the esterification of carboxylic acid and alcohol. Easily absorb moisture, soluble in ethanol and water. White crystalline powder, solubility in water >: 20g/ 100ml, storage and transportation temperature 2-8℃, in inert gas.

Extended data:

Mechanism of edc:

In organic synthesis, edc is a commonly used dehydrating agent, which promotes the formation of amides and esters. N- hydroxybenzotriazole or N- hydroxysuccinimide is often added in the reaction, which can improve the yield and reduce the occurrence of side reactions. Edc can also react with amines to form guanidine.

The by-product of the reaction is mainly N- acyl urea produced by rearrangement of O- acyl isothiourea. Using solvents with low dielectric constant (such as dichloromethane and chloroform) can reduce the formation of N- acyl urea.

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