Six Dynasties Museum has one basement and three floors above ground, with the highest point of 24 meters. The gate of the Six Dynasties Museum is in the south, facing Changjiang Road and echoing the new Jiangsu Provincial Art Museum.

From the beginning of the 3rd century to the end of the 6th century, the six dynasties, namely, Song Dynasty, Qi Dynasty, Liang Dynasty and Chen Dynasty, were collectively called "Six Dynasties". Nanjing, the ancient capital of the Six Dynasties, is known as the "ancient capital of the Six Dynasties". At that time, Nanjing in the Six Dynasties was also the first city in the world with a population of more than one million, and it was called "the two centers of world classical civilization" together with the ancient Roman city. The Six Dynasties is an extremely important period in the history of China's cultural development, and it is also an important chapter in the history of Nanjing city and capital city. The splendid culture of the Six Dynasties left many cultural allusions and historical sites for Nanjing. Therefore, the core theme of the museum exhibition is to show the elegance of "Oriental Metropolis". There are four exhibitions: the ancient capital of the Six Dynasties, the eternal romance, the elegance of the Six Dynasties and the talents of the Six Dynasties. Through historical exhibitions, people exhibitions, fine works exhibitions and story exhibitions, we can learn from "city", "people", "things" and "beauty"